XFD700 Anti-human CD45 Antibody *UCHL1, XFD700 Same Structure to Alexa Fluor™ 700*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.07 |
Extinction coefficient (cm -1 M -1) | 192000 |
Excitation (nm) | 696 |
Emission (nm) | 719 |
Quantum yield | 0.251 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Overview | SDSProtocol |
Other names CD45RO | Clone UCHL1 | Host mouse | Isotype Mouse IgG2a, κ | Reactivity human | Application Flow Cytometry (FACS); Fluorescence Imaging | Correction Factor (260 nm) 0.00 | Correction Factor (280 nm) 0.07 | Extinction coefficient (cm -1 M -1) 192000 | Excitation (nm) 696 | Emission (nm) 719 | Quantum yield 0.251 |
UCHL1 is an anti-human monoclonal antibody that forms an immune complex with the CD45ro antigen. CD45ro (sometimes called CD45RO) is a 180 kD transmembrane glycoprotein that is found on the surface of cells like granulocytes, macrophages and NK cells. In certain organisms, CD45 plays a role in the upregulation of protein kinase activity, is a positive regulator of tumor necrosis factor production and is a negative regulator of protein kinase activity. Additionally, it has been associated with key biological processes like dephosphorylation, particularly protein dephosphorylation. CD45 acts in key cellular pathways, for example, the regulation of receptor signaling pathway via JAK-STAT, B cell receptor signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. From a research standpoint, it is of biological interest due to its association with important macromolecules/ligands like Src kinases, p56lck and p59fyn. CD45 is a very popular antibody target, with over 54000 publications in the last decade. CD45ro is often used in flow cytometry applications as a phenotypic marker for differentiation of cell types, specifically in the study of inhibitory molecules and immunology. This antibody was purified through affinity chromatography and conjugated to XFD700 (ex/em = 696/719 nm). XFD700 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 700 (Alexa Fluor® is the trademark of ThermoFisher).
Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.00 |
Correction Factor (280 nm) | 0.07 |
Extinction coefficient (cm -1 M -1) | 192000 |
Excitation (nm) | 696 |
Emission (nm) | 719 |
Quantum yield | 0.251 |
Antibody Finder
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Conjugation
We provide custom conjugation services for this antibody (eg. labeling of cd45ro antibody with HRP). A list of available labels can be found in the table below:
For additional information about custom conjugations, please consult our services page here.
AF | AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750 |
Proteins | HRP, Alkaline Phosphatase, Streptavidin |
Tandems | APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5 |
Small Molecules | Biotin |
Traditional Dyes | FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5 |
iFluor | 350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7 |
mFluor | UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780 |
For additional information about custom conjugations, please consult our services page here.
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
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Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?