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XFD700 Anti-human CD8 Antibody
HIT8a
The HIT8a monoclonal antibody binds to human CD8a, a 32 - 34 kD member of the Ig superfamily often located on the surface of dendritic cells, t cells and thymocytes. CD8 is a component of important cellular pathways, namely, the transmembrane receptor protein tyrosine kinase signaling pathway and cell surface receptor signaling pathway. From a research standpoint, it is of biological interest due to its association with key macromolecules/ligands like Lck and MHCI. CD8 is a very popular antibody target, with over 125000 publications in the last decade. CD8a is frequently used in flow cytometry applications as a phenotypic marker for differentiation of cell types, particularly in the study of immunology. This antibody was purified through affinity chromatography and conjugated to XFD700 (ex/em = 696/719 nm). XFD700 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 700 (Alexa Fluor® is the trademark of Thermo Fisher).
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with XFD700 Anti-human CD8 Antibody *HIT8a. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific R4-A channel.
(Top) Spectral emission profiles generated using four spatially offset lasers (355 nm, 405 nm, 488 nm, and 640 nm). Each laser produced a distinct emission pattern, and their combination yielded the composite spectral signature. (Bottom) Flow cytometry analysis of whole blood stained with XFD700 Anti-human CD8 Antibody *HIT8a. The fluorescence signal was monitored using an Aurora spectral flow cytometer in the  specific R4-A channel.
datasheet
Datasheet
COA
CatalogSize
Price
Quantity
100801A0100 tests
Price
100801A1500 tests
Price
 
Physical properties
Molecular weightN/A
Solventwater
Antibody properties
Other namesT8, Leu2
CloneHIT8a
HostMouse
IsotypeIgG1
ReactivityHuman
ApplicationFlow Cytometry (FACS), Fluorescence Imaging
Spectral properties
Correction factor (260 nm)0.00
Correction factor (280 nm)0.07
Extinction coefficient (cm -1 M -1)
192000
Excitation (nm)696
Emission (nm)719
Quantum yield
0.25
1
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
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Page updated on April 4, 2026