Calcium flux assays are preferred methods in drug discovery for screening F protein coupled recepters (GPCRs)
Screen Quest™ Fluo-8 No Wash Calcium Assay Kits provides homogeneous fluorescence-based assays for detecting intracellular calcium mobilization across a broad spectrum of biological targets. The characteristics of its long wavelength, high sensitivity and 200 times fluorescence enhancement (when it forms a complex with calcium) make Fluo-8 NW an ideal indicator for measurement of cellular calcium.
The signal intensity and well-to-well uniformity of Fluo-8 NW is compared with
Fluo-3 AM,
Fluo-4 NW yields the brightest signal, more the 2 fold brighter than
Fluo-4 AM, and 4 times brighter than Fluo-3 AM. The Screen Quest™ Fluo-8 NW Calcium Assay Kits provide an optimized assay method for monitoring GPCRs and calcium channels. Unlike Fluo-3 AM and Fluo-4 AM, both of which require 37°C for optimal cell loading, the Screen Quest™ Fluo-8 NW requires a less temperature-dependent cell loading property, giving similar results either at room temperature or 37°C. This characteristic makes the Screen Quest™ Fluo-8 NW Calcium Assay Kits more robust for HTS applications.
The Screen Quest™ Fluo-8 NW Calcium Assay kit has been optimized for broad range of instruments to give maximum performance with GPCR and calcium channel tagets.
The Screen Quest™ Fluo-8 NW Calcium Assay Kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. Its ability to generate a very large signal from the weak and robust assay systems enables researchers to have multiple assay chemistries for different receptor and calcium channel targets.