AssayWise Letters 2019, Vol. 8(1)
Introducing CytoCite™ Fluorometer with Cloud-Integration

ReadiView™ Biotin: All-In-One Biotinylation & Quantification of Biotin Labeling

Fundamentals of Flow Cytometry

ReadiUse™ Lyophilized Phycobiliproteins

Buccutite™ Conjugation Technology

AssayWise Letters Vol. 8(1)

Featured Article
Introducing CytoCite™ Fluorometer with Cloud-Integration
Introducing CytoCite™, the very first cloud-integrated handheld fluorometer powered by Device Cloud. The CytoCite™ Fluorometer is a compact, green-channel fluorometer designed for highly sensitive fluorescence-based quantitative analysis. It provides users with the simplicity and robustness to accelerate their downstream applications such as sequencing, PCR, and immunoassays. Read More...



ReadiView™ Biotin: All-In-One Biotinylation & Quantification of Biotin Labeling
The high binding affinity of streptavidin for biotin molecules is one of the strongest known non-covalent interactions. By taking advantage of this preferential interaction, researchers in multiple scientific fields conjugate molecules of interest to one or the other of these two, to be able to better measure and purify their chosen materials. The selection and adjustment of reagents, buffers, incubation times and other variables must be optimized experimentally, and require assessments to measure the degree of success. The ReadiView™ Biotin series allows the researcher to perform the biotinylation and quantification processes and avoid the common but lengthy HABA assay step.

Fundamentals of Flow Cytometry
The flow cytometer is a powerful machine that permits extremely fast multi-parameter analysis of biological samples. The use of flow cytometry is gaining popularity in many research fields as the costs associated with the machinery diminish and the technology steadily improves. Scientific applications as challenging as downstream cell cycle analysis can now be precisely measured, assisted with new probes such as the CytoTell™ family of dyes, which can be used to visualize up to 9 generations for most cell lines.

ReadiUse™ Lyophilized Phycobiliproteins
Phycobiliproteins perform extremely well as fluorescent tags for multiple applications in research science, due to their high absorbance coefficients and quantum yields over a wide spectral range. These qualities have made them widely used in flow cytometry, but commercial phycobiliproteins require a tedious and lengthy dialysis procedure to remove the unwanted compounds and must be used quickly even when stored in ideal conditions. After our ammonia sulfate-free lyophilization treatment, the pre-activated phycobiliprotein storage maximum improves from the prior 1 month maximum (for refrigerated native pigment) up to a full year even stored at RT.

Buccutite™ Conjugation Technology
AAT Bioquest has developed an optimized and robust Buccutite™ crosslinking technique. Buccutite™ crosslinking technology provides a simplistic and efficient approach for labeling proteins with PE, APC or tandem dyes. It can be completed in half the time and with less stringent parameters yielding highly stable and easy to handle conjugated proteins.