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Can I measure NADPH without lysing my cells?
Posted August 21, 2019
NADH AssayNADP/NADPH ratioNADHNADPNADPHNADWestern Blotcell lysiscell assayemission wavelengthexcitation wavelengthfluorescence imagingfluorimetriclive cell analysislive cell assaymultiplex
Answer
Most traditional blots and protein assays involve cell lysates, but in recent years, new probes have been developed that will permit the visualization of NADP+/NADPH levels in living cells. Intracellular probes will enable the researcher to take measurements without a cell lysing step. For multiparametric experiments, choose a fluorophore that is at an extreme wavelength, to reduce spectrum overlap and the necessity for data correction.
Table 1. Product information for Cell Meter™ Intracellular NADH/NADPH assay kits.
| Product Name | Sensor | Color | Instrument | Unit Size | Cat No. |
| Cell Meter™ Intracellular NADH/NADPH Fluorescence Imaging Kit | JZL1707 NAD(P)H Sensor | Red Fluorescence | Fluorescence Microplate Reader Fluorescence Microscope | 100 Tests | 15290 |
| JJ1902 NAD(P)H Sensor | Deep Red Fluorescence | 100 Tests | 15295 | ||
| Cell Meter™ Intracellular NADH/NADPH Flow Cytometric Analysis Kit | JZL1707 NAD(P)H Sensor | Red Fluorescence | Flow Cytometer | 100 Tests | 15291 |
| JJ1902 NAD(P)H Sensor | Deep Red Fluorescence | 100 Tests | 15296 |
For assistance in choosing appropriate dyes for simultaneous measurement applications, see AAT Bioquests' online spectrum comparison tool in 'Additional Resources' below.
Additional resources