AAT Bioquest

Are NADH and ROS related?

Posted August 26, 2019


Reactive Oxygen Species, or ROS, are a potentially damaging biochemical byproduct of multiple cellular pathways that involve redox reactions, with the most prominent contributors being the energy pathways facilitated by the coenzymes NAD+ and NADP. The reduced forms of these coenzymes are used for detoxification of ROS, and the various ROS themselves are used in a temporary fashion for intra- and inter-cellular signaling. There are currently ~400 known redox reactions in the cell that involve NAD+/NADH, and another ~30 for the phosphorylated variation NADP/NADPH. The far-reaching influence of these molecules makes their measurement and visualization useful even in seemingly unrelated experiments. 

For more information about biochemical measurements of cellular health, see 'Additional Resources' below. 

Additional resources

ROS Measurement and Detection

NAD(P)/NAD(P)H Products

Cell Viability