Name: Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*
Brand: AAT Bioquest
SKU: 15280
Price: 415 USD
Availability: InStock

Amplite® Fluorimetric NAD Assay Kit

Blue Fluorescence

Detection Principle: Directly measures NAD using the Quest Fluor™ NAD Probe.
Sensitivity: Detects NAD down to 30 nM in a 100 µL assay.
Specificity: The probe reacts selectively with NAD and shows little response to NADH.
Workflow: Compatible with 96- or 384-well microplates and high-throughput screening.
Instrument Compatibility: Fluorescence detection at Ex/Em 420/480 nm.

<p>Comparison of NAD and NADH response</p>

Protocol

SDS

Catalog	Size	Price	Quantity
15280	200 Tests	Price

Overview

Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells. Quantifying the generation or consumption of these factors is an important method to monitor the enzyme-mediated reaction or screening the modulator or substrate of these enzyme reactions. There are several kits on the market to quantify NADH or total NAD/NADH amount, but detection NAD generation in the presence of large excess amount of NADH has been quite challenging to date because NAD has its absorption peak at 259 nm and does not fluorescence, making the measurement unpractical. Amplite® Fluorimetric NAD Assay Kit provides a sensitive and rapid detection of NAD. The kit directly measure NAD using Quest Fluor™ NAD reagent, our newly developed NAD sensor. The proprietary probe used in this kit reacts only with NAD to generate a product that fluorescence at a specific excitation and emission spectra range, and has little response to NADH. This kit can detect as little as 30 nM NAD in a 100 µL assay volume, and monitor 0.3% NAD generation in the presence of excess amount of NADH. This assay can be performed in a convenient 96-well or 384-well microtiter-plate format and can be used in high-throughput screening.

Citations

View all 61 citations: Citation Explorer

Domain compositions of Arabidopsis Toll/Interleukin-1 Receptor/Resistance domain-containing TX14 proteins affect localization and induction of the hypersensitive response

Authors:

Lim, Jaebeom and Yeon, Jinouk and Lee, Sang Ho and Song, Sang-Kee and Yi, Hankuil

Journal:

Journal of Experimental Botany (2026): erag334

Decrease of NAD+ Inhibits the Apoptosis of OLP T Cells via Inducing Mitochondrial Fission

Authors:

Zhang, Zhuo-Yu and Wang, Fang and Zhou, Gang

Journal:

Journal of Inflammation Research (2025): 1091--1106

Oral Delivery of Nanoparticle Urolithin A Normalizes Cellular Stress and Improves Survival in Mouse Model of Cisplatin-induced AKI

Authors:

Zou, Dianxiong and Ganugula, Raghu and Arora, Meenakshi and Nabity, Mary B and Sheikh-Hamad, David and Kumar, MNV Ravi

Journal:

American Journal of Physiology-Renal Physiology (2019)

Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation

Authors:

Yang, Fei and Heit, Caitlin and Inglis, Debra L

Journal:

Fermentation (2017): 61

Pyrroloquinoline Quinone, a Redox-active o-Quinone, Stimulates Mitochondrial Biogenesis by Activating SIRT1/PGC-1α Signaling Pathway

Authors:

Saihara, Kazuhiro and Kamikubo, Ryosuke and Ikemoto, Kazuto and Uchida, Koji and Akagawa, Mitsugu

Journal:

Biochemistry (2017)

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Fluorescence microplate reader
Excitation	420 nm
Emission	480 nm
Recommended plate	Solid black

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Page updated on October 14, 2026

Component A: Quest Fluor™ NAD Probe	1 bottle (5 mL)
Component B: Assay Solution	1 bottle (5 mL)
Component C: Enhancer Solution	1 bottle (3.5 mL)
Component D: NAD Standard	1 vial (332 µg)