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Can SYPRO Ruby be reused?
Posted August 16, 2021
Gel ElectrophoresisDNA and RNA QuantitationNucleus
Answer

Yes, SYPRO Ruby can be reused but there are a few limitations you should be aware of.

SYPRO Ruby can be re-used only up to two times without affecting its detection sensitivity. Using a twice-used stain reduces the signal intensity up to 2.5 fold. Increasing the staining volume to 100ml is advisable to improve detection sensitivity when reusing the stain.

Additional resources
Optimized conditions for diluting and reusing a fluorescent protein gel stainGel ElectrophoresisHelixyte™ Gold Nucleic Acid Gel Stain *10,000X DMSO Solution*

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