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Can antibodies for flow cytometry be used for fluorescence microscopy?
Posted April 24, 2020
Antibodies and ProteomicsFluorescenceSpectral Flow Cytometryflow cytometryfluorescence microscope
Answer

Many antibodies may be suitable for use in both flow cytometry and fluorescence microscopy. The best method is to check whether the antibodies have been validated for use in the intended applications.

Additional resources
Weller M. G. (2018). Ten Basic Rules of Antibody Validation. Analytical chemistry insights, 13, 1177390118757462. doi:10.1177/1177390118757462

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