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How does spectral flow cytometry manage autofluorescence?
Posted June 22, 2020
Answer
Autofluorescence is treated as an independent parameter in spectral flow cytometry, whose spectral patterns can be identified by spectral unmixing. Therefore, it can be removed during data analysis, simplifying gating and allowing for low-expressing targets to be more readily identified.
Additional resources
Fundamentals of Flow Cytometry Cell Meter™ FITC-Annexin V Binding Apoptosis Assay Kit *Optimized for Flow Cytometry* Picot, J., Guerin, C. L., Le Van Kim, C., & Boulanger, C. M. (2012). Flow cytometry: retrospective, fundamentals and recent instrumentation. Cytotechnology, 64(2), 109-130. Nolan, J. P., & Condello, D. (2013). Spectral flow cytometry. Current protocols in cytometry, 63(1), 1-27.