Below is a brief protocol on how to use coelenterazine h for in vivo imaging of mice:

1. Make a 5 mg/mL coelenterazine stock solution in Ethanol + 2% v/v 3N HCl
2. Add 20 µL of the above coelenterazine stock solution to 130 µL of PBS
3. For intravenous injections, the recommended dosage is 15 – 100 µg/mouse (25 – 150 µL/mouse).
4. Inject your mice with the recommended dosage using a 25 gauge needle or higher.
5. Image your mice as soon as possible for peak signal with intravenous injection