What is the best way to administer D-Luciferin potassium salt for in vivo bioluminescent imaging?

Should I inject it intravenously, intramuscularly or intraperitoneally?

You can administer D-Luciferin potassium salt all three ways intravenously, intramuscularly or intraperitoneally. The recommended dosage is 150 mg/kg in 10 mL/kg volume (15mg/mL solution). See the detailed protocol below for administering D-Luciferin intravenously.