Does EDTA interfere with your Cell Meter™ Colorimetric Antioxidant Activity Assay Kit (Cat # 15900)?
Posted April 2, 2019
I’d like to measure antioxidant activity in human blood samples, but the samples were collected using EDTA as anticoagulant.
No, EDTA does not interfere with our Cell Meter™ Colorimetric Antioxidant Activity Assay. As high as 5 mM EDTA was tested in this assay, and no interference was observed.
Cell Meter™ Colorimetric Antioxidant Activity assay relies on the ability of antioxidants to inhibit the oxidation of ABTS (2, 2’-azino-bis (3-ethylbenzothiazoline6-sulfonic acid) to ABTS+ by HRP and H2O2. ABTS+, is a soluble green color chromogen, that can be monitored spectrophotometrically at 405 nm. Under testing conditions, the absorbance suppressed in the test samples is proportional to the concentrations of antioxidants in those samples. Trolox, a water-soluble vitamin E analog, is used as a standard antioxidant reference.