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AAT Bioquest

Does PCR use polymerase?

Posted February 8, 2022


Answer

Yes, PCR uses polymerase.

PCR (polymerase chain reaction) is a laboratory technique used to amplify and generate many copies of a specific DNA region. Polymerase is a type of enzyme that synthesizes new strands of DNA, using the original strands as templates. DNA polymerase is an essential component for this process.

The most commonly used DNA polymerase in PCR is Taq polymerase.

Additional resources

Polymerase Chain Reaction (PCR)

PhosphoWorks™ Fluorimetric Pyrophosphate Assay Kit *Blue Fluorescence*