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How do I calculate PCR efficiency?
Posted November 30, 2020
DNA and RNAChemical ReagentsClassic Dyes
Answer

PCR efficiency can be calculated based on the slope of the standard curve. Slopes ranging between -3.1 and -3.6 typically equate to reaction efficiencies that range between 90 and 110%, respectively. The following equation is used to calculate PCR efficiency: 

E = -1+10(-1/slope)

 

Additional resources
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*Polymerase Chain Reaction (PCR)

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