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How do I calculate PCR efficiency?
Posted November 30, 2020
Answer
PCR efficiency can be calculated based on the slope of the standard curve. Slopes ranging between -3.1 and -3.6 typically equate to reaction efficiencies that range between 90 and 110%, respectively. The following equation is used to calculate PCR efficiency:
E = -1+10(-1/slope)
Additional resources
A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.
6-TET phosphoramidite [5'-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*