What is the difference between Rhodamine 123 and Rhodamine B?
Posted October 1, 2021
- Green fluorescent mitochondrial dye
- Molecular formula C21H17ClN2O3
- Excitation maxima 507 nm
- Emission maxima 534 nm
- Soluble in ethanol 1 mg/mL
- Is cell membrane permeant
- Stains mitochondria in living cells
- Widely used in flow cytometry studies involving mitochondrial membrane potential and bacteria. Also used in biochemistry to inhibit mitochondrion function.
- Bright red fluorescent xanthene dye
- Molecular formula C28H31ClN2O3
- Excitation maxima 568 nm
- Emission maxima 583 nm
- Soluble in water and methanol
- Functions as a water tracer fluorescent
- Widely used as a fluorescent dye in confocal microscopy and for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
Rhodamine B is used in biology as a staining fluorescent dye to demonstrate acid-fast organisms, notably mycobacterium. Rhodamine B is also a commonly used dye for industrial purposes such as printing and dyeing in textile, paper, paints, leathers, and so on.