Widely used in flow cytometry studies involving mitochondrial membrane potential and bacteria. Also used in biochemistry to inhibit mitochondrion function.
Widely used as a fluorescent dye in confocal microscopy and for cross staining 1-N-methylamino-5-isocyanonaphthalene (MICAN)
Rhodamine B is used in biology as a staining fluorescent dye to demonstrate acid-fast organisms, notably mycobacterium. Rhodamine B is also a commonly used dye for industrial purposes such as printing and dyeing in textile, paper, paints, leathers, and so on.