AAT Bioquest

Which rhodamines are used in biological research laboratories?

Posted October 1, 2021


Rhodamine B, Rhodamine 123, and Rhodamine 6G are the three main rhodamine dyes used in biological research laboratories in applications such as fluorescence microscopy, ELISA, fluorescence correlation spectroscopy, and flow cytometry.

  • Rhodamine B is used as a staining fluorescent dye.
  • Rhodamine 123 is used as a tracer dye for membrane transport and also as an inhibitor of mitochondrial function.
  • Rhodamine 6G is a fluorescence tracker and it can also be used as an organic laser dye to study probes as it has a high quantum yield for fluorescence.
Additional resources

Synthesis of a Far-Red Photoactivatable Silicon-Containing Rhodamine for Super-Resolution Microscopy

Rhodamines and Rhodamine Derivatives

Rhodamine 800 *CAS 137993-41-0*