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Rhodamine B *CAS 81-88-9*

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Physical properties
Molecular weight479.01
Spectral properties
Absorbance (nm)541
Extinction coefficient (cm -1 M -1)1060001
Excitation (nm)546
Emission (nm)567
Quantum yield0.71, 0.432
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.71, 0.432
Rhodamine B (also called Rhodamine 610, Basic Violet 10, or C.I. 45170) is used as a fluorescent staining dye, sometimes in combination with auramine O. The auramine-rhodamine stain is used to demonstrate acid-fast organisms, notably Mycobacterium; Rhodamine B is tunable around 610 nm when used as a laser dye.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Rhodamine B *CAS 81-88-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM208.764 µL1.044 mL2.088 mL10.438 mL20.876 mL
5 mM41.753 µL208.764 µL417.528 µL2.088 mL4.175 mL
10 mM20.876 µL104.382 µL208.764 µL1.044 mL2.088 mL

Molarity calculator

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Spectral properties

Absorbance (nm)541
Extinction coefficient (cm -1 M -1)1060001
Excitation (nm)546
Emission (nm)567
Quantum yield0.71, 0.432

Product Family

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Rhodamine 800 *CAS 137993-41-0*682704-
Rhodamine 6G *CAS 989-38-8*5255481160001
Sulforhodamine B *CAS 3520-42-1*559577-
Rhodamine 110 *CAS 13558-31-1*50052280000
Rhodamine 123 *CAS 62669-70-9*50852885200
Rhodamine aldehyde [5-TAMRA aldehyde]55257890000



View all 1 citations: Citation Explorer
Optothermal crystallization of hard spheres in an effective bidimensional geometry
Authors: Ruzzi, Vincenzo and Baglioni, Jacopo and Piazza, Roberto
Journal: The Journal of Chemical Physics (2023)


View all 39 references: Citation Explorer
Adsorption behavior of rhodamine B on Rhizopus oryzae biomass
Authors: Das SK, Bhowal J, Das AR, Guha AK.
Journal: Langmuir (2006): 7265
Controllable size, shape and morphology of molybdic acid self-aggregated with rhodamine B to construct functional material
Authors: Guan ZS, Zhang Y, Zhang Q, Li DX.
Journal: J Colloid Interface Sci (2006): 113
Interaction of a mitochondrial membrane potential-sensitive dye, rhodamine 800, with rat mitochondria, cells, and perfused hearts
Authors: Jilkina O, Kong HJ, Hwi L, Kuzio B, Xiang B, Manley D, Jackson M, Kupriyanov VV.
Journal: J Biomed Opt (2006): 14009
Relationship between excretion clearance of rhodamine 123 and P-glycoprotein (Pgp) expression induced by representative Pgp inducers
Authors: Kageyama M, Fukushima K, Togawa T, Fujimoto K, Taki M, Nishimura A, Ito Y, Sugioka N, Shibata N, Takada K.
Journal: Biol Pharm Bull (2006): 779
Fluorescent plasma nanocomposite thin films containing nonaggregated rhodamine 6G laser dye molecules
Authors: Barranco A, Groening P.
Journal: Langmuir (2006): 6719
Fluorescence resonance energy transfer quenching method for determination of arsenic with acridine orange-rhodamine B
Authors: Liu BS, Gao J, Liu ZC, Yu LN, Yang DC, Yang GL.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2006): 306
Resonance scattering effect of rhodamine dye association nanoparticles and its application to respective determination of trace ClO2 and Cl2
Authors: Jiang ZL, Zhou SM, Liang AH, Kang C, He X.
Journal: Environ Sci Technol (2006): 4286
Complex formation between rhodamine B and poly(sodium 4-styrenesulfonate) studied by 1H-NMR
Authors: Moreno-Villoslada I, Gonzalez R, Hess S, Rivas BL, Shibue T, Nishide H.
Journal: J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2006): 21576
Ab initio study of optical properties of rhodamine 6G molecular dimers
Authors: Gavrilenko VI, Noginov MA.
Journal: J Chem Phys (2006): 44301
Determination of trace lead by solid substrate room temperature phosphorescence enhancing method based on heavy atom effect and dissoluble manganese supramolecule containing rhodamine 6G luminescent particles
Authors: Lin X, Wu RH, Li XJ, Chen L, Lin QW, Gao WY, Zheng AF, Liu JM, Zhu GH, Huang XM.
Journal: Spectrochim Acta A Mol Biomol Spectrosc. (2006)