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What is the difference between Cy5 and Cy5.5?
Posted July 20, 2021

Answer

Cy5 and Cy5.5 are both cyanine dyes with several differences between them.

Basis for comparisonCy5Cy5.5
Type of dyeFar-red fluorophorenear-infrared (IR) fluorescence-emitting dye
Molecular weight667.54767.66
Molecular formulaC36H42N3BF4O4C44H46N3BF4O4
Absorbance maximum646 nm675 nm
Emission maximum662 nm694 nm
HydrophobicityHighly hydrophobicHydrophilic due to the presence of four sulfonate groups
ApplicationsUsed in Fluorescence Resonance Energy Transfer (FRET), Fluorescence Polarization (FP), and Time Resolved- Fluorescence Resonance Energy Transfer (TR-FRET).Used to label DNA oligos for use as hybridization probes in other applications such as Fluorescent In-Situ Hybridization (FISH).