AAT Bioquest

Which cyanine dyes can be used for in vivo imaging applications?

Posted July 27, 2021


In vivo fluorescence imaging uses a sensitive camera to detect fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the near-infrared (NIR) region are generally preferred, such as small indocarbocyanine dyes. Near infrared (NIR) cyanine-based dyes, Cy5, Cy5.5 and Cy 7, are most commonly used for in vivo applications.

Additional resources

Near-infrared Molecular Probes for In Vivo Imaging


Cyanine 5 monoacid [equivalent to Cy5® acid]