AAT Bioquest

Can Cy3 be used in flow cytometry?

Posted July 27, 2021


Yes, Cy3 can be used in flow cytometry, however it is more appropriate for use in applications where a smaller dye is required. This is because it does not give the fluorescence intensity comparable to that of larger dyes such as PE (Phycoerythrin) or APC (Allophycocyanin).

Additional resources

Flow Cytometry: An Overview

PE and APC

Tide Fluor™ 3 acid [TF3 acid] *Superior replacement for Cy3*