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Posted June 5, 2019

What is the main difference in each detection probe

ROS peroxynitrite anion ONOO- flow cytometry fluorescence microscope

Answer

The catalog 16316 kit measures peroxynitrite anion ONOO- in solution, the probe is not cell-permeable. However, both 16315 and 16317 kits are for intracellular peroxynitrite anion measurement, the probe is cell permeable. Kit 16315 is optimized for fluorescence microscopy and fluorescence microplate reader, and kit 16317 is optimized for flow cytometry. All the 3 kits are measured at excitation and emission with 490 and 525 nm (FITC filter sets).

Additional resources

Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*Amplite™ Fluorimetric Peroxynitrite Quantification Kit *Green Fluorescence*Cell Meter™ Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*

Can I treat my cells before adding the master reaction mix for ROS kit 22900 What are the differences between your fluorimetric peroxynitrite assays (Cat# 16315, 16316 and 16317)?Does Cell Meter™ Fluorimetric Mitochondria Superoxide Activity Assay Kit support multiplex assays for detection of mitochondrial superoxide?Do you have substrates for proteasome 20S subunits, such as beta 1C, 2C, 5C, 1i, 2i, and 5i?Can I add MitoROS dyes to my cell growth media?

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