Can I add MitoROS dyes to my cell growth media?

Q: Can I add MitoROS dyes to my cell growth media?

Yes, you can add MitoROS™ 580 and MitoROS™ 520 to cell growth media. We've tested both dyes in DMEM containing 5% serum, and they performed well. Furthermore, by adding the dye to the growth media it increases the dye incubation time (1-2 hours).

Posted June 12, 2019

Can I use it with growth media containing serum?

ROS

cell assay

flow cytometry

Answer

Yes, you can add MitoROS^™580 and MitoROS^™520 to cell growth media. We've tested both dyes in DMEM containing 5% serum, and they performed well. Furthermore, by adding the dye to the growth media it increases the dye incubation time (1-2 hours).

Additional resources

Protocol for Cell Meter™ Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*

Protocol for Cell Meter™ Fluorimetric Intracellular Superoxide Detection Kit *Flow Cytometry *

Protocol for Cell Meter™ Fluorimetric Intracellular Superoxide Detection Kit *Fluorescence Microplate Reader*

MitoROS™ 580 *Optimized for Detecting Reactive Oxygen Species (ROS) in Mitochondria*