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Apoptosis assay selection for GFP and RFP transfected tumor cells?

Posted June 11, 2019


We are trying to examine the effect of tumor cells transfected with genes on apoptosis; the glioma is transfected with mirna lentivirus (green fluorescence) and then transfected with target gene (red fluorescence) lentivirus.
We would like to study the effect of these factors on apoptosis; Which apoptotic kits are suitable for our experiment?

Answer

Please try these kits: 

Cat# 22793, 22832 for Cell MeterTM Phosphatidylserine Apoptosis Assay (optimized for fluorescene and flow cytometry, respectively)

Cat# 17548 for Necrosis Assay 

Cat# 22803, 22802 for Mitochondrion Membrane Potential Assay

Additional resources

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*

Nuclear Blue™ DCS1

Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*

Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Reader*