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AAT Bioquest

Apoptosis assay selection for GFP and RFP transfected tumor cells?

Posted June 11, 2019


We are trying to examine the effect of tumor cells transfected with genes on apoptosis; the glioma is transfected with mirna lentivirus (green fluorescence) and then transfected with target gene (red fluorescence) lentivirus.
We would like to study the effect of these factors on apoptosis; Which apoptotic kits are suitable for our experiment?

Answer

Please try these kits: 

Cat# 22793, 22832 for Cell MeterTM Phosphatidylserine Apoptosis Assay (optimized for fluorescene and flow cytometry, respectively)

Cat# 17548 for Necrosis Assay 

Cat# 22803, 22802 for Mitochondrion Membrane Potential Assay

Additional resources

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit

Cell Meter™ Phosphatidylserine Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*

Nuclear Blue™ DCS1

Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry*

Cell Meter™ NIR Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Reader*