Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Do you have substrates for proteasome 20S subunits, such as beta 1C, 2C, 5C, 1i, 2i, and 5i?

Posted April 2, 2019


Answer

Yes, we offer a set of R110 substrates for monitroing the activity of proteasomes at different subsites. Protease activity is measured by monitoring the R110 liberation over time using either a flow cytometer, fluroescence microscope or fluorescent microplate reader at Ex/Em = 490/520 nm.

List of R110 substrates (subsite; R110 substrate):

beta1c, Z-LLE-R110 (Cat#13466)

beta2c, Ac-KQL-R110 (Cat#13465)

beta5c, Ac-WLA-R110 (Cat#13468)

beta1i, Ac-PAL-R110 (Cat#13467)

beta2i, Ac-KQL-R110 (Cat#13465)

beta5i, Ac-ANW-R110 (Cat#13455)

 

Additional resources

(Z-LLE)2R110

(Ac-KQL)2R110

(Ac-WLA)2R110

(Ac-PAL)2R110

(Ac-ANW)2R110