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Do you have substrates for proteasome 20S subunits, such as beta 1C, 2C, 5C, 1i, 2i, and 5i?
Posted April 2, 2019
flow cytometerflow cytometryfluorescence imagingfluorescence microplate readerfluorescence microscopefluorescent plate reader
Answer

Yes, we offer a set of R110 substrates for monitroing the activity of proteasomes at different subsites. Protease activity is measured by monitoring the R110 liberation over time using either a flow cytometer, fluroescence microscope or fluorescent microplate reader at Ex/Em = 490/520 nm.

List of R110 substrates (subsite; R110 substrate):

beta1c, Z-LLE-R110 (Cat#13466)

beta2c, Ac-KQL-R110 (Cat#13465)

beta5c, Ac-WLA-R110 (Cat#13468)

beta1i, Ac-PAL-R110 (Cat#13467)

beta2i, Ac-KQL-R110 (Cat#13465)

beta5i, Ac-ANW-R110 (Cat#13455)

 

Additional resources
(Z-LLE)2R110(Ac-KQL)2R110(Ac-WLA)2R110(Ac-PAL)2R110(Ac-ANW)2R110

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