logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Do you provide any dyes similar to CFSE that can track cells or monitor cell proliferation?
Posted June 13, 2019

Answer

We provide CytoTell™ dyes in a variety of excitation-emission profiles that monitor cell proliferation using either a fluorescence microplate reader, fluorescence microscope or flow cytometer.

Similar to CFSE, CytoTell™ dyes are cell-permeable indicators that covalently bind to intracellular lysine residues and other amine sources located on cytoplasmic proteins.  This covalent coupling ensures that CytoTell™ dyes are not transfered to adjacent cells and are well-retained in cells for several generations. As cells divide, CytoTell™ dyes are distributed equally between daughter cells, and each new generation of cells is marked by a fluorescence intensity half that of its parents.

Compared to CFSE, CytoTell™ dyes have minimal cytotoxicity and better cellular retention. CytoTell™ dyes, other than CytoTell™ Green, are excellent for multicolor applications with either GFP cell lines or iFluor 488™-labeled antibodies.