We provide CytoTell® dyes in a variety of excitation-emission profiles that monitor cell proliferation using either a fluorescence microplate reader, fluorescence microscope or flow cytometer.

Similar to CFSE, CytoTell® dyes are cell-permeable indicators that covalently bind to intracellular lysine residues and other amine sources located on cytoplasmic proteins.  This covalent coupling ensures that CytoTell® dyes are not transfered to adjacent cells and are well-retained in cells for several generations. As cells divide, CytoTell® dyes are distributed equally between daughter cells, and each new generation of cells is marked by a fluorescence intensity half that of its parents.

Compared to CFSE, CytoTell® dyes have minimal cytotoxicity and better cellular retention. CytoTell® dyes, other than CytoTell® Green, are excellent for multicolor applications with either GFP cell lines or iFluor 488®-labeled antibodies.