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Do I need to deparaffinize my paraffin-embedded tissue sections before staining F-actin with Phalloidin-iFluor® conjugates? Can you provide a deparaffinization protocol?
Posted April 2, 2019
Actin iFluor dyesCell AssayFixationF-actinFixativesfluorescence imagingfluorescence microscopephalloidin
Answer
Yes, in order to stain F-actin with any fluorescent phalloidin conjugate you must first deparaffinize (remove paraffin) your paraffin-embedded tissue sections. To deparaffinize your tissue section:
- Warm slides to 57 °C for 5 minutes.
- Immerse sections in 2 changes of xylenes, 5 minutes each.
- Immerse sections in 100%, 95%, and then 70% ethanol, 5 minutes each.
- Wash 2 times in 1X PBS, 5 minutes each.
- Proceed to F-actin labeling procedure.
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