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AAT Bioquest

How do I compute the population doubling time of my cell culture?

Posted December 18, 2020


Answer

Use the following equation to calculate the cell doubling time:

 

Where:

  • Nt is the number of cells at time t
  • N0 is the number of cells initially at time 0
  • t is time (days)
  • gr is the growth rate
Additional resources

Growth rates of normal and abnormal human mammary epithelia in cell culture

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Optimized for 405 nm Violet Laser Excitation*

Cell Proliferation Assays