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How do I compute the population doubling time of my cell culture?
Posted December 18, 2020
Cellular ProcessesCell Cycle and ProliferationPhysiological Probes
Answer

Use the following equation to calculate the cell doubling time:

 

Where:

  • Nt is the number of cells at time t
  • N0 is the number of cells initially at time 0
  • t is time (days)
  • gr is the growth rate
Additional resources
Growth rates of normal and abnormal human mammary epithelia in cell cultureCell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Optimized for 405 nm Violet Laser Excitation*Cell Proliferation Assays

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