AAT Bioquest

How do I make my cell culture immortal?

Posted December 15, 2020


Cells can induce immortality spontaneously through random mutation or it can be introduced via genetic modifications. A common technique for inducing immortality is to introduce a viral gene (e.g. tumor suppressor genes) that supersedes the cell cycle. For example, adenovirus type 5 DNA was used to immortalize HEK293 cells.

Additional resources

Cell Cycle and Proliferation

Cell Meter™ Fluorimetric Live Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*

Immortalized cells and one oncogene in malignant transformation: old insights on new explanation