How do I prepare a spin column?
Posted April 2, 2019
I'd like to use a 1 mL spin column, such as a Sephadex® G-25, BioGel® P-6 DG Media or other desalting column to purify 100 µL of a reaction mixture. Can you provide a sample protocol?
Answer
Below is a sample procedure on how to set up and use a 1 mL spin column for purification of 100 µL test conjugate:
Fill with beads Drain the solution, top dry Buffer exchange using ~4 mL PBS buffer (pH 7.2 - 7.4) Spin column at 2000X for 2 minutes Exchange current 15 mL tube for a brand new one Load sample mixture directly to the top of the desalting column Add 10 µL of PBS buffer (pH 7.2 - 7.4) Spin column at 2000X for 5 minutes Collect elution Measure the concentration using a spectrophotomer / / Copyright © 2025 AAT Bioquest, Inc. All Rights Reserved.