Add 500uL DMSO/vial
Incubate cells with a dye solution at room temperature or 37 °C for 30 min, protected from light.
Prepare a 1X dye working solution by diluting the 500X DMSO stock solution at 1 to 500 in Hanks and 20 mM Hepes buffer (HHBS) or the buffer of your choice.