AAT Bioquest

How does NGS work?

Posted February 16, 2022


NGS (next generation sequencing) is a massively parallel sequencing technology that’s used to determine the order of nucleotides in entire genomes or targeted regions of DNA or RNA. Next generation sequencing builds upon “first generation sequencing” technologies to yield more accurate and cost-effective sequencing results.

The NGS process involves fragmenting DNA/RNA into multiple pieces, adding adapters, sequencing the libraries, and reassembling them to form a genomic sequence. This process sequences millions of fragments in a parallel fashion, improving accuracy and speed while lowering the cost of sequencing as compared to first-generation sequencing

Additional resources

A Next-Generation Sequencing Primer—How Does It Work and What Can It Do?