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How does the autophagy marker in your Cell Meter™ Autophagy Assay Kit (Cat#23000) work?
Posted June 13, 2019
What makes this kit different from your Cell Meter™ Autophagy Fluorescence Imaging Kit (Cat#23001)?
Answer
The autophagy marker preferentially accumulates in autophagic vacuoles due to a combination of ion trapping and specific interactions with membrane lipids.
The Autophagy Super Blue™ maker available in Cat#23001 is an enhanced autophagy probe that generates a stronger more intense fluorescence signal and higher signal-to-background ratio. It’s improved sensitivity for staining autophagosomes versus cell lysosomes greatly reduces background interference.