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AAT Bioquest

How long does tissue culture take?

Posted December 4, 2020


Answer

The duration it takes for tissue culturing largely depends upon the type of tissue or cell sample (i.e. plant or animal). Plant tissue cultures take several weeks to complete. It takes approximately 2 – 3 weeks for the development of a callus, which in turn takes several weeks for shoots and roots to develop. These newly formed plantlets are then acclimatized for a period of time to ensure proper hardening before being transferred to soil.

Culturing cells from animal tissue is generally less time-consuming and depending upon the generation time cell cultures can be ready in just a few days. However, some cell types such as neurons take weeks to mature, anywhere from 3 to 6 weeks

Additional resources

The scientific roots of modern plant biotechnology

Cell Cycle and Proliferation