AAT Bioquest

How many biotin binding-sites are there per streptavidin molecule?

Posted April 20, 2021


Wild-type streptavidin is a tetramer with four identical subunits, each containing a high affinity binding site for biotin (K D = 10 -15 M) . Monovalent streptavidin is a tetramer with 3 inactive subunits and one subunit that binds biotin with wild-type affinity.

Additional resources

A monovalent streptavidin with a single femtomolar biotin binding site

Biotin *CAS 58-85-5*