I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?

Q: I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?

Protonex™ Red 600, SE (Cat No. 21208) is an amine-reactive pH-dependent dye that can be used to label proteins, antibodies and other biomolecules to study phagocytosis and endocytosis. Below is a sample protocol for labeling cancer cells with pH-dependent dye Protonex™ Red 600, SE . Suspend cells at a density of 1 x 106 cells/mL in PBS buffer or HH buffer. Prepare 5 mg/mL Protonex™ Red 600, SE stock solution in DMSO. Add Protonex™ Red 600, SE to your cell suspension for a final concentration of 5~10 µg/mL Incubate at 37°C for one hour. Wash cells twice with PBS buffer or HH buffer, and then suspend cells in 1 mL complete medium or PBS buffer. Cells are ready for phagocytosis experiment.

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Accepted Answer

Protonex&trade; Red 600, SE (Cat No. 21208) is an amine-reactive pH-dependent dye that can be used to label proteins, antibodies and other biomolecules to study phagocytosis and endocytosis.
Below is a sample protocol for labeling cancer cells with pH-dependent dye Protonex&trade; Red 600, SE .

Suspend cells at a density of 1 x 106 cells/mL in PBS buffer or HH buffer.
Prepare 5 mg/mL Protonex&trade; Red 600, SE stock solution in DMSO.
Add Protonex&trade; Red 600, SE to your cell suspension for a final concentration of 5~10 &micro;g/mL
Incubate at 37&deg;C for one hour.
Wash cells twice with PBS buffer or HH buffer, and then suspend cells in 1 mL complete medium or PBS buffer.
Cells are ready for phagocytosis experiment.

I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?

Posted January 2, 2020