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Is Hoechst cell permeable?
Posted May 6, 2021
NucleusDNA and RNA
Answer

Yes, Hoechst is cell permeable. Both, Hoechst 33258 and Hoechst 33342 are cell permeable, however Hoechst 33342 is considerably more cell permeable because of the addition of a lipophilic ethyl group. Hoechst 33342 is usually the preferred choice for staining living cells.

Additional resources
SiR-Hoechst is a far-red DNA stain for live-cell nanoscopyHoechst 33342 *Ultrapure Grade*Hoechst 33258 *CAS 23491-45-4*

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