AAT Bioquest

Is Hoechst toxic to cells?

Posted May 6, 2021


Hoechst is minimally toxic to cells especially when used at very low concentrations. It is the preferred dye for staining live cells because of its lower cytotoxicity as compared to other DNA stains.

Additional resources

Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy

Hoechst 33258 *CAS 23491-45-4*

Hoechst 33342 *Ultrapure Grade*