What are sticky and blunt ends of DNA molecules?
Posted July 22, 2020
A straight cut of restriction enzymes generates blunt ends, where both strands terminate in a base pair. Blunt ends are also called non-cohesive ends, since there is no unpaired DNA strand fleeting at the end of DNA.
The sticky ends, a.k.a. cohesive ends, have unpaired DNA nucleotides on either 5’- or 3’- strand, which are known as overhangs. These overhangs are most often generated by a staggered cut of restriction enzymes. Sticky ends are generally more desired in cloning technology where a DNA ligase is used to join two DNA fragments into one, because the yield and specificity of ligation using sticky ends is significantly higher that with blunt ends.