AAT Bioquest

What are the advantages and disadvantages of serum-free media in cell culture?

Posted July 22, 2020


The main advantages of using serum-free media are:

  • Increased definition and more reproducible formulations, allowing for more convincing and consistent research results.
  • Easier purification and downstream processing.
  • Lower risk of contamination with bacteria, fungi or virus.
  • Elimination of time-consuming batch tests.

However, there are also some drawbacks for serum-free media:

  • Requirement for cell type-specific media formulation.
  • High demand for reagent purity.
  • Slower growth rate.
Additional resources

Cell Cycle and Proliferation

Assay development services

Berg, J. M., Tymoczko, J. L., & Stryer, L. (2008). Biochemistry (Loose-Leaf). Macmillan.

Yao, T., & Asayama, Y. (2017). Animal?cell culture media: History, characteristics, and current issues. Reproductive medicine and biology, 16(2), 99-117.

Butler, M. (2004). Animal cell culture and technology. Taylor & Francis.