What are the applications of RT-PCR?

RT-PCR (Reverse Transcriptase PCR) is a vital step in several RNA-based assays.

It is widely used in research laboratories to study and measure gene expression. For example, it is used in experiments to distinguish exons from introns.

Scientists also use RT-PCR to identify changes in a gene or chromosome to diagnose diseases such as cancer, monitor response to cancer therapy, and improve prognosis. 

Another application of RT-PCR is the study of viruses such as HIV, hepatitis C, and Influenza Virus A, whose genomes are composed of RNA. This can help in diagnosing and monitoring infections caused by these viruses.

RT-PCR is also used in the diagnosis of certain genetic diseases such as Lesch-Nyhan syndrome, which is caused by an error in the HPRT1 gene.