RT-qPCR (Quantitative Reverse Transcription PCR) has a wide range of applications including:

• Relative and absolute quantification of gene expression
• Detection of pathogens such as viruses for the diagnosis of infectious diseases
• Validation of DNA microarray results
• miRNA (microRNA) and noncoding RNA analysis
• Mutation detection
• Validation of RNAi to study loss of function of selective genes
• Variation analysis including SNP discovery and validation
• Detection of genetically modified organisms (GMOs)
• Counting viral, bacteria, or fungal loads