AAT Bioquest

What are the differences between PCR and DNA sequencing?

Posted March 11, 2022


PCR and DNA sequencing are two vital techniques in molecular biology. Here’s a look at the differences between these two techniques:


  • Is a technique used to duplicate specific DNA regions in vitro
  • Is often used in the process of DNA sequencing
  • Millions or even billions of copies of the target DNA region are produced during this process
  • The objective is to produce sufficient quantity of DNA for the next process, which is sequencing
  • The process uses dNTPs (Deoxynucleotide Triphosphates)

DNA Sequencing:

  • Is a technique used to determine the sequence of the bases in DNA
  • The process enables researchers to visualize the correct order of the bases in a particular DNA fragment
  • The objective is to determine the precise order of the nucleotides in a given DNA fragment
  • The process uses ddNTPs (Dideoxynucleotide triphosphates) to terminate strand formation
Additional resources

Comparison of next-generation sequencing systems

MagaDye™ 4 Color Sanger Sequencing Terminator Kit