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What are the differences between PCR and DNA sequencing?
Posted March 11, 2022
Physiological ProbesDNA and RNA QuantitationCellular Processes
Answer
PCR and DNA sequencing are two vital techniques in molecular biology. Here’s a look at the differences between these two techniques:
PCR:
  • Is a technique used to duplicate specific DNA regions in vitro
  • Is often used in the process of DNA sequencing
  • Millions or even billions of copies of the target DNA region are produced during this process
  • The objective is to produce sufficient quantity of DNA for the next process, which is sequencing
  • The process uses dNTPs (Deoxynucleotide Triphosphates)
DNA Sequencing:
  • Is a technique used to determine the sequence of the bases in DNA
  • The process enables researchers to visualize the correct order of the bases in a particular DNA fragment
  • The objective is to determine the precise order of the nucleotides in a given DNA fragment
  • The process uses ddNTPs (Dideoxynucleotide triphosphates) to terminate strand formation
Additional resources
Comparison of next-generation sequencing systemsMagaDye™ 4 Color Sanger Sequencing Terminator Kit

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