PCR and DNA sequencing are two vital techniques in molecular biology. Here’s a look at the differences between these two techniques:

PCR:

• Is a technique used to duplicate specific DNA regions in vitro
• Is often used in the process of DNA sequencing
• Millions or even billions of copies of the target DNA region are produced during this process
• The objective is to produce sufficient quantity of DNA for the next process, which is sequencing
• The process uses dNTPs (Deoxynucleotide Triphosphates)

DNA Sequencing:

• Is a technique used to determine the sequence of the bases in DNA
• The process enables researchers to visualize the correct order of the bases in a particular DNA fragment
• The objective is to determine the precise order of the nucleotides in a given DNA fragment
• The process uses ddNTPs (Dideoxynucleotide triphosphates) to terminate strand formation