AAT Bioquest

What are the common types of alternative splicing?

Posted July 22, 2022


There are five different types of alternative splicing: exon skipping, constitutive splicing, mutually exclusive splicing , alternative 3’ or 5’ splice site, and intron retention.

  • Exon skipping is when an exon is spliced out of the primary RNA strand or kept; this is the most common type of alternative splicing.
  • Constitutive splicing is the process of removing introns from pre-mRNA and sticking the exons together to form a mature mRNA.
  • Mutually exclusive splicing is a type of splicing in which only one of two or more potential exons is spliced into the mature mRNA. 
  • The alternative 3’ splice acceptor site is used to change the 5’ downstream exon. The alternative 5’ splice donor site is used to change the 3’ upstream exon.
  • Intron retention is an alternative splicing mode whereby introns are spliced out as an intron or retained in mature mRNAs. 
Additional resources

Mechanism of alternative splicing and its regulation

Cell Navigator® Live Cell RNA Imaging Kit *Green Fluorescence*