What are the differences between a fluorophore and a chromophore?
Posted January 29, 2021
Answer
Fluorophores and chromophores are chemical substances that are responsible for various visible effects in compounds. A look at the salient features of fluorophores and chromophores offers a clear indication of the differences between the two.
Fluorophore
- Is a fluorescent chemical compound capable of re-emitting light upon light excitation
- Absorbs light energy of a certain wavelength and re-emits the energy as a longer wavelength.
- Capable of absorbing light ranging from UV to blue light
- Capable of emitting light in higher wavelengths
- Capable of emitting high energy
- The exciting and emitting wavelengths overlap
- Responsible for the fluorescent property of a molecule
- Emission is temperature-dependent
- Types include intrinsic fluorophores, which occur naturally in a sample, and extrinsic fluorophores, which are manually added to a sample to change the sample’s spectral properties
- Main applications include as a dye or staining agent, as a tracer in fluids, and as a substrate for enzymes.
Chromophore
- Is part of a molecule that is responsible for giving the molecule its color
- Capable of absorbing light from UV to the visible region
- Capable of emitting light in the visible range
- The exciting and emitting wavelengths do not overlap
- Capable of emitting low energy
- Responsible for giving a molecule its specific color
- Emission is not temperature-dependent
- Types include metal complex and conjugated π bond system chromophores
- Main applications include food colorings, fabric dyes, and pH indicators.
Additional resources
Mechanism of Chromophore Assisted Laser Inactivation Employing Fluorescent Proteins