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What are the differences between ion exchange chromatography and affinity chromatography?
Posted December 23, 2021
Antibodies and ProteomicsBioconjugationAntibody and Protein LabelingProtein to Protein Conjugation
Answer

Ion exchange chromatography:

  • Is a form of liquid chromatography that’s used to separate charged particles
  • Is based on the interaction between the column and the target molecule that has an opposite charge to that of the stationary phase surface
  • Examples: inorganic cations and anions, organic ions, and proteins

 Affinity chromatography:

  • Is used to separate components in a mixture
  • Is based on the interactions between the target and the stationary phase surface
  • Can be used to separate charged or uncharged components in a mixture
  • Examples: enzymes, substrates, DNA, antigens, proteins, and antibodies
Additional resources
Affinity chromatography: A review of trends and developments over the past 50 yearsBuccutite™ Rapid Protein Crosslinking Kit *Microscale Optimized for Crosslinking 100 ug Antibody Per Reaction*

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