What are the differences between next-generation sequencing (NGS) and qPCR?
Posted February 18, 2022
|Basis of Differences||Next-Generation Sequencing (NGS)||qPCR|
|Discovery power or ability to identify novel variants||Offers considerably higher discovery power enabling detection of novel variations. Also offers higher sensitivity enabling quantification of rare variants and transcripts||Very poor discovery power. Is capable of detecting only known sequences.|
|Scalability and throughput||Offers higher sample throughput – a single NGS experiment is capable of identifying variants across numerous target regions with single base resolution||Offers lower scalability - is more effective for lower target numbers. Even then, the workflow can be unwieldy for multiple targets. Enables interrogation of a limited set of variants.|
|Cost effectiveness||Less cost-effective for sequencing lower numbers of targets (1–20 targets).||More cost effective when the number of target regions is less than 20 targets and when the study aims to screen or identify known variants.|
Whether NGS or qPCR is better for you will depend on a variety of factors including the number of samples, study goals, total amount of sequence in the target regions, and budgetary considerations.