Types of polymerase chain reaction (PCR):

• Real-Time PCR (quantitative PCR or qPCR) – in which DNA molecules are tagged using fluorescent dye, which is used to monitor and quantify PCR products in real-time
• Reverse-Transcriptase (RT–PCR) – creates complementary DNA (cDNA) by reverse transcribing RNA to DNA using reverse transcriptase
• Multiplex PCR – uses a number of primers to multiply multiple fragments in a single DNA sample
• Nested PCR – after the initial 25-35 PCR cycles, an additional PCR is conducted using new primers “nested” within the original primers, which reduces the risk of unwanted products
• Hot Start PCR – in which heat is used to denaturate antibodies that are used to inactivate Taq polymerase
• Long-range PCR – longer ranges of DNA are formed by using a mixture of polymerases
• Assembly PCR – longer DNA fragments are aplified by using overlapping primers
• Asymmetric PCR – only one strand of the target DNA is amplified
• In situ PCR – PCR that takes place in cells, or in fixed tissue on a slide