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What are the different types of PCR?

Posted January 21, 2021


Answer

Types of polymerase chain reaction (PCR):

  • Real-Time PCR (quantitative PCR or qPCR) – in which DNA molecules are tagged using fluorescent dye, which is used to monitor and quantify PCR products in real-time
  • Reverse-Transcriptase (RT–PCR) – creates complementary DNA (cDNA) by reverse transcribing RNA to DNA using reverse transcriptase
  • Multiplex PCR – uses a number of primers to multiply multiple fragments in a single DNA sample
  • Nested PCR – after the initial 25-35 PCR cycles, an additional PCR is conducted using new primers “nested” within the original primers, which reduces the risk of unwanted products
  • Hot Start PCR – in which heat is used to denaturate antibodies that are used to inactivate Taq polymerase
  • Long-range PCR – longer ranges of DNA are formed by using a mixture of polymerases
  • Assembly PCR – longer DNA fragments are aplified by using overlapping primers
  • Asymmetric PCR – only one strand of the target DNA is amplified
  • In situ PCR – PCR that takes place in cells, or in fixed tissue on a slide
Additional resources

https://www.aatbio.com/catalog/polymerase-chain-reaction-pcr

https://www.aatbio.com/products/cyber-green-sybr-green-20x-aqueous-pcr-solution?unit=17591

https://www.aatbio.com/products/cyber-green-sybr-green-10-000x-aqueous-pcr-solution?unit=17592