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What are the recommended ranges of input RNA amount in reverse transcription reactions?
Posted March 4, 2022
Cell Structures and OrganellesNucleusDNA and RNA QuantitationPhysiological Probes
Answer

A snapshot of the recommended ranges of input RNA amount in reverse transcription reactions:

10 pg–5 μg for Total RNA

10 pg–0.5 μg for Poly(A) RNA

0.01 pg–0.5 μg for Specific RNA

Additional resources
Eleven Golden Rules of Quantitative RT-PCRHelixyte™ Green dsDNA Quantifying Reagent *200X DMSO Solution*

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