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AAT Bioquest

What do I need to perform whole genome sequencing?

Posted February 16, 2022


Answer

As whole genome sequencing (WGS) starts with DNA, you would first need to extract DNA from a cell or tissue sample. Samples may be obtained from bone marrow, saliva, epithelial cells, hair with hair follicle, plant leaves, and seeds and other DNA-containing cells. Because these biological samples contain a full copy of DNA, as little as 100 ng of DNA provides sufficient genetic material for performing whole genome sequencing.

You will also need a library preparation kit and adapters to prepare libraries. Adapters typically come with barcodes or indexes that help to identify the sample or specific molecules in the sample.

Additional resources

A step-by-step beginner’s protocol for whole genome sequencing of human bacterial pathogens

7-Deaza-7-Propargylamino-3'-azidomethyl-dATP